CD4 抗体
Quick Overview for CD4 抗体 (ABIN114149)
抗原
See all CD4 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody recognizes Mouse CD4.
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纯化方法
- Purified
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亚型
- IgG2a
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应用备注
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Flow Cytometry (see Protocols).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- FLOW CYTOMETRY ANALYSIS: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of this
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限制
- 仅限研究用
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浓度
- 0.2 mg/mL
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缓冲液
- PBS, 0.09 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- CD4
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别名
- CD4
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背景
- The CD4 (L3/T4) antigen appears to be expressed by the helper/inducer subset of murine T cells and by delayed hypersensitivity T cells but not by cytotoxic T cells or their precursors. CD4 (L3/T4) and CD8a (Ly 2) have been shown to be present on mutually exclusive T cells in the peripheral lymphoid organs but the thymus contains cells expressing both CD4 (L3/T4) and CD8a (Ly 2).Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
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基因ID
- 12504
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NCBI登录号
- NP_038516
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UniProt
- P06332
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途径
- TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
抗原
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